Immunoglobulin heavy chain gene rearrangement in follicular lymphoma

Written by Mezei Tibor, Horváth Emőke, Tóth Erika, Csernák Erzsébet, Pap Zsuzsanna, Pávai Zoltán, Szentirmay Zoltán, Jung János

Molecular biology studies used in the diagnosis of lymphomas are being represented especially by clonality analysis and the study of non-random cromosomial translocations. The aim of our study was to evaluate the usefullness of clonality assessment in paraffin embedded archived bioptic material. 19 cases from the archives of the Pathology Department of the Emergency Clinical Hospital Targu Mures were included in the study. Molecular biology studies consisted of conventional and real time PCR. The primers used were choosen according to the BIOMED-2 study. In 3 cases the obtained DNA was unsuitable for molecular studies, two of these being lymphoma cases and one lymph node with reactive lesions. 68,42% of the cases (13 cases) proved to be monoclonal, 15,78% (2 cases) oligoclonal and 5,26% (1 case) policlonal. Molecular biology studies bring further support or dismiss the malignant nature of the lesions. This is especially valid in cases where the tissue architecture is highly suggestive of reactive lesion. In these cases, clonality assessment may confirm the malignant nature of the lesion by proving the monoclonal origin of the tumour cells.

 

Keywords: follicular lymphoma, clonality assessment, IgH gene rearrangement, PCR


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