Fermentation kinetics sudy of the Saccharomyces Bayanus culture in the presence of pharmaceutical adjuvants used for immobilisation

Written by Dudutz Gyöngyi, Csép Katalin, Gál Emese

Alcoholic fermentation is one of the possibilities to transform biomass resulted from plant photosynthesis into energy transporters. Yeast transforms simple sugars in alcohol, which is one of the least polluting energy resources of the future. The aim of the present study was to find the optimal and most economic working conditions for the process of etylic alcohol production. Research has been done on a yeast species - Saccharomyces Bayanus (Hefix 2000) - selected specifically for alcoholic fermentation obtained from the Biotechnology Research Institute Bay Zoltan from Szeged, Hungary. During fermentation variable factors have been determined, like the product and substrate concentration variation and the variation of the number of live and total inocculated yeast cells. We have calculated the constants that characterize the kinetic processes of fermentation, the variation of live cells and biomass in % as well as the yield. Alcoholic fermentation was repeated in the presence of three auxiliary pharmaceutic substances: polivynil-pirrholydone, Tween 80, and Na-alginate, by maintaining all the other conditions constant. We have also determined the critical mycellar concentration of the three studied auxiliary susbtances to correlate them with the kinetics and yield of fermentation. In conclusion, from the point of view of product yield and biomass quantity, the best results have been obtained with kolidon, rarely cited in the literature as generally Na-alginate is used for immobilization.

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