Benzodiazepines (BZD) are among the most frequently used psychotropic drugs, that is the reason why the elaboration of new analysis methods for these substances is a permanent necessity. The aim of the study is to elaborate a thin-layer chromatography (TLC) method for the separation of 8 frequently used BZD derivates. We managed to separate the 8 BZD using a Kieselgel 60 F254 stationary phase, a mixture ethyl acetate-cyclohexanconcentrated ammonia (50+40+0.5) as mobile phase and UV detection at 254 and 366 nm. Also we managed to separate by TLC the degradation products in acidic medium of the BZD mentioned above.